Sequence Id:
Paste template sequence below Or upload sequence file:       
Due to performance reasons a sequence can be maximally 2000 bp long. If the submitted sequence is bigger, select an included region < 2000 bp.
Included Region (start,length):  {  }   
Please visit About for Primer3 and Primer3Plus citation requests.

Primer3Prefold uses for folding the software UNAFold, a successor of mfold. License conditions require that you cite the following article if you use Primer3Prefold:

Markham, N. R. & Zuker, M. (2008) UNAFold: software for nucleic acid folding and hybriziation. In Keith, J. M., editor, Bioinformatics, Volume II. Structure, Function and Applications, number 453 in Methods in Molecular Biology, chapter 1, pages 3–31. Humana Press, Totowa, NJ. ISBN 978-1-60327-428-9. [Abstract] [Full Text] [PDF]